High Performance Liquid Chromatography Runing paeoniflorin Pill.docVIP

 PAGE \* MERGEFORMAT 6 High Performance Liquid Chromatography Runing paeoniflorin Pill [Keywords:] Runing pills paeoniflorin high-performance liquid chromatography Runing pills from TPG, Zhejiang Fritillaria, Angelica, etc. 12 flavor extracts of Chinese herbal medicines made from fine powder, and the pills. With Shugan blood circulation, Ruanjian Sanjie the efficacy for the treatment of breast pain are blocks, irregular menstruation, or less, the color purple into blocks and breast hyperplasia. Runing pills in order to control the quality assurance of clinical drug effects, this experiment as an indicator of paeoniflorin, using high performance liquid chromatography of its content with satisfactory results. An experimental material Aglient 1100 high-performance liquid chromatography, G1314A UV measuring instruments, 77251 manual injector, Zhejiang University N2000 chromatography data workstation; paeoniflorin Chemical Reference (by the Chinese pharmaceutical and biological products to offer Lot: 110736  200321 Size: for content determination); acetonitrile for chromatography pure; other reagents were analytical pure. 2 Methods and Results 2.1 Chromatographic conditions Column: ReliaSil  C18 (4.6 mm * 250 mm, 5 μ m); mobile phase: acetonitrile -0.1% phosphoric acid (eighty-seven past one p.m.); flow rate: 0.7 mL / min; The detection wavelength: 233 nm; column temperature: at room temperature. 2.2 Preparation of reference substance solution obtained precision that paeoniflorin reference substance 6.25 mg, buy 5 mL Liang Ping, add methanol dissolved and diluted to the scale, shake, precision drawing 1 mL, home 5 mL Liang Ping, add methanol dissolves and diluted to the scale, shake, or have (each 1 mL contains 0.25 mg of paeoniflorin). 2.3 Preparation of solution for the test items to take this product 2 g, precision that set, and placing a plug conical bottle, precision adding methanol 100 mL, said that given the weight, place 2 h, ultrasonic treatme