HPLC determination in different places Erigeron scutellarin content.docVIP

 PAGE \* MERGEFORMAT 10 HPLC determination in different places Erigeron scutellarin content Author: Liu Peng, Zhou Jun, Xiang-Yang Qin, WANG Qiao-Feng, Li Ye [Keywords:], scutellarin Determination of scutellarin in Erigeron breviscapus from different places by HPLC [Abstract] AIM: To develop a HPLC method to determinate the proportion of scutellarin in Erigeron breviscapus from different places of production. METHODS: The analytical column was a ZODBAXC18 (4.6 mm * 150 mm) filled with a 5 μ m stationary phase. The mobile phase consisted of methanol (0.02 mol / L) phosphate buffer solution [pH 5.2 (35:65)]. The flowrate was 0.8 mL / min. The injection volume was 20 μ L. The Detector was set at 335 nm. The column temperature was 25 ℃ . RESULTS: The calibration curve was linear over the range of 0.74-3.70 μ g (r = 0.999 6). The average recovery of the method was 97.24%, RSD 0.82% (n = 6). CONCLUSION: The proposed method would be used in quantitative analysis of scutellarin in Erigeron breviscapus. The method is convenient, economical and accurate with good reproducibility. [Keywords] scutellarin; HPLC; Erigeron breviscapus [Abstract] Objective: To determine the different areas of Erigeron scutellarin content. Methods: Using high-performance liquid chromatography with ZODBAXC18 (4.6 mm * 150 mm, 5 μ m) as column, mobile phase was methanol -- 0.02 mol / L, phosphate buffer pH 5.2 (35:65), detection wavelength of 335 nm, flow rate 0.8 mL / min, column temperature 25 ℃ , other than the standard method of Erigeron in scutellarin content. Results: Erigeron B elements in the range of 0.74 ~ 3.70 μ g within the peak area showed good linear relationship, r = 0.999 6, scutellarin average recovery of 97.24%, RSD of 0.82% (n = 6). Conclusion: This method is reliable and high accuracy and good reproducibility for Erigeron in scutellarin by HPLC. [Keywords:] scutellarin; high-performance liquid chromatography; Erigeron 0 Introduction The Compositae Erigeron Erigeron