HPLC Determination of Wei Kang Capsules paeoniflorin.docVIP

 PAGE \* MERGEFORMAT 7 HPLC Determination of Wei Kang Capsules paeoniflorin Author: Wang Qiang, JIA, WEI Zhong-bao, QI Yan-juan [Abstract] Objective: To explore the Wei Kang Capsules paeoniflorin by HPLC. METHOD: C18 column (250mm * 4.6mm), acetonitrile - water - phosphoric acid (16:84:0.1) as mobile phase; detection wavelength 230nm; flow rate 1ml.min; nm. Results: The linear range of paeoniflorin 0.0916 ~ 1.832μ g, r = 0.9999, average recovery was 97.48%, RSD is 1.52%. Conclusion: This measurement method is accurate, reproducible, can be used as the preparation of quality control methods. [Keywords:] Wei Kang Ling Capsule; paeoniflorin; high-performance liquid chromatography; determination Wei Kang Ling Capsule is a compound consisting of eight taste of Chinese medicine preparations, with Rougan and stomach, Sanyu bleeding, urgency and pain relief, to saprophytic new role. For acute and chronic gastritis, gastric ulcer, erosive gastritis, duodenal ulcer and bleeding embolism. In order to effectively control the quality of the formulations of paeoniflorin were determined [1,2]. An instrument and reagent Shimadzu LC-10AT vp HPLC, SPD-10A vp UV detector, 7725i manual injector, Zhejiang University, N2000 chromatography data workstations, paeoniflorin chemical reference substances (drugs and biological products from China provided, grant No. :736-9407, specification: for assay). Wei Kang Ling Capsule (medicine provided by the Heilongjiang sunflower), the use of pure and analytically pure reagents were chromatography. 2 Methods and Results 2.1 Chromatographic conditions and system suitability test column: ReliaS-il-C 18 (250mm * 4.6mm); mobile phase: acetonitrile - water - phosphoric acid (16:84:0.1); detection wavelength: 230nm; Sensitivity: 0.05AUFS; flow rate: 1ml.min; column temperature: room temperature; theoretical plate number calculated by paeoniflorin peak should not be less than 2000. 2.2 Preparation of reference substance solution, said precis