HPLC Determination of wheat germ oil capsules in the nutritional content of vitamin E.docVIP

 PAGE \* MERGEFORMAT 7 HPLC Determination of wheat germ oil capsules in the nutritional content of vitamin E [Abstract] Objective To determine the nutrition of wheat germ oil capsules in the vitamin E content in approach. Methods HPLC, column was Agilent ZORBAX ODS column, methanol  acetonitrile (volume ratio 25:75) as mobile phase, flow rate 1.0 mL / min, detection wavelength 290 nm, column temperature is 40 ℃ . The results of vitamin E in the 32.9 ~ 291.1 μ g / mL within the linear relationship between the average recovery was 99.9%, RSD of 1.0% (n = 6). Conclusion This method is simple, accurate and can effectively control the quality of the preparation. [Keywords:] nutritious wheat germ oil capsules; vitamin E; high-performance liquid chromatography Abstract: Objective To establish an HPLC method for determining the content of vitamin E in wheat germ oil. Methods An Agilent ZORBAX ODS column was used to analysis the sample by using methanol  acetontril (25:75) as mobile phase. The flow rate was 1.0 mL * min-1 and detective wavelength 290 nm.Results The standard curve for vitamin E was linear in the concentration range of 32.9 ~ 291.1 μ g / mL. The average recovery was 99.9% with RSD 1.0% (n = 6). Conclusion This method is simple, accurate and reliable for the quality control of wheat germ oil. Keywords:: wheat germ oil vitamin E; HPLC Of wheat germ oil capsules is a Sino-US cooperation nutrition Amway (China) Commodity Co., Ltd. products, whose main components are unsaturated fatty acids, vitamin E, choline, plant sterols, there are a variety of glutathione and other trace elements, with anti-infertility, anti-aging, promote human metabolism, enhance physical strength and memory, lower cholesterol, prevent atherosclerosis, improve blood circulation, strengthen the immune system function. Its main active ingredient of vitamin E, vitamin E needs to be measured through the sample saponification, extraction, washing, concentration and other steps were