HPLC method for the determination of liver and syrup content of Sal B_0.docVIP

 PAGE \* MERGEFORMAT 7 HPLC method for the determination of liver and syrup content of Sal B Of: high pass Peng, Wen-Qiang Li, Speeding [Abstract] Objective To establish the liver and syrup in the determination of Sal B. Methods using high performance liquid chromatography of methanol - acetonitrile -1.5% formic acid (30:10:60) as mobile phase, flow rate 1.0 mL / min, detection wavelength was 286 nm. the results of the linear range of 0.302 4 ~ 1.512 μg (r = 0.999 9), the average recovery was 99.79%, RSD = 0.23%. Conclusion The method is efficient, rapid, sensitive, and can be used as the liver effective method of quality control syrup. [Keywords:] the liver syrup, Sal B, HPLC, determination Abstract: Objective To set up a method for determining the content of Salvianolic acid B in Qianggan Syrup. Methods HPLC was adopted. A mixture of methanol-acetonitrile-1.5% formic acid (30:10:60) served as the mobile phase with a flow rate of 1.00 mL / min. The detection wavelength was 286 nm. Results The linear range was 0.145 ~ 1.747 μg, r = 0.999 9. The average recovery rate was 99.79%, RSD = 0.23%. Conclusion The method is highly effective, quick and sensitive, and suitable for quality control of the preparation. Keywords:: Qianggan Syrup, Salvianolic acid B, HPLC, content determination The liver and syrup from the capillaries, Salvia, Astragalus, Radix, Angelica, white peony root, Codonopsis, Hawthorn, Gentiana, 16 herbs such as liquorice, whose main function is to heat and dampness, spleen and blood, Qi Jieyu for the treatment of chronic hepatitis, cirrhosis, fatty liver, toxic hepatitis. Sal B (Salvianolic acid B, SA-B) is a water-soluble active ingredient in Salvia. Danshen as the Prince of prescription drugs, and their contents directly affect the control of the efficacy of the preparation, so we try to use strong HPLC method for the determination Sal B in the liver syrup content, through methodological study found that the method is accurat