IL1B +3954 - Taqpolymorphism and susceptibility to chronic periodontitis.docVIP

 PAGE \* MERGEFORMAT 11 IL1B +3954 / Taqpolymorphism and susceptibility to chronic periodontitis Of: Han Bing, Gou Jian weight, Xi Chen, Sheng-Bin Li [Abstract] Objective To investigate IL 1B +3954 / Taq gene polymorphism and chronic periodontitis susceptibility. Methods Han population of 116 subjects, including 66 patients with moderate to severe chronic periodontitis (CP) patients and 50 cases of periodontal healthy controls, peripheral blood leukocytes extracted DNA, using standard polymerase chain reaction restriction fragment length polymorphism (PCR RFLP) method, the IL 1B +3954 / Taq genotype testing, compare the The detection rate of genotype differences. Results IL 1B +3954 / Taq sites are dominated A1/A1 genotype, CP Group A1/A2 genotype detection rates and the healthy control group had no statistical difference. Conclusions IL 1B +3954 / Taq allele 2 may be in Chinese Han population genetic susceptibility to severe chronic periodontitis independent. [Keywords:] periodontitis; interleukin-1; single nucleotide polymorphism; genotype; genetic susceptibility ABSTRACT: Objective To study whether specific IL 1B +3954 / Taq genotype alleles are associated with chronic periodontitis (CP) in Chinese of the Han nationality. Methods CP group consisted of 66 patients while healthy controls group consisted of 50 subjects. Anti coagulated peripheral blood sample was obtained from each subject and genomic DNA was extracted from each sample. Single nucleotide polymorphisms (SNPs) at IL 1B +3954 / Taq were analyzed by standard polymerase chain reaction restriction fragment length polymorphisms (PCR RFLP) assay. Results In the control group, higher numerical values of the A1/A1 genotype were observed for the IL 1B +3954 / Taq SNPs (84.0%), and the A2 + genotype was present in 16% of this sample. Statistical analysis revealed no significant difference between the two groups. Conlusion There is no evidence in our study supporting the association betwe