In the developing brain of rat neuronal nitric oxide synthase expression.docVIP

 PAGE \* MERGEFORMAT 15 In the developing brain of rat neuronal nitric oxide synthase expression [Abstract] Objective To investigate the use of immunohistochemistry of neuronal nitric oxide synthase in rat brain development in rats. Methods randomly selected E12, E15, E18, P0, P5 and P10 of the SD rats, the application immunohistochemical method to detect cerebral cortex, striatum and hippocampus the density of nNOS positive cells. Results E12, E15 of rat brain and E18 no nNOS-positive cells, cortex and striatum P0, P5 and P10 of nNOS positive cells were seen. nNOS-positive cell density in P0, P5 and P10 of decreased pairwise comparison, there was significant difference (P lt;0.05). P0 occasionally of nNOS positive cells in the hippocampus, P5 and P10 of both of nNOS positive cells are more visible, but the density of nNOS positive cells between P5 and P10 was no significant difference of (Pgt; 0.05). Conclusion embryonic rat brain nNOS staining was negative, the cerebral cortex and striatum of nNOS after birth density of positive cells decreased, hippocampal nNOS positive cell density increased. [Keywords:] neuronal nitric oxide synthase (nNOS) immunohistochemistry of brain development in rats ABSTRACT: Objective To observe the expression of neuronal nitric oxide synthase (nNOS) during the growth of brain in rats with immunohistochemical method. Methods Fetal and newborn rats were randomly selected, and divided into 6 groups according to developmental stages of E12, E15, E18 , P0, P5 and P10. The sections were stained by nNOS immunohistochemical staining. The density of nNOS immunoreactive cells was collected and measured by image gathering and analysis software in the sections of cerebral cortex, striatum and hippocampus. Results nNOS immunoreactive cells were not found at telencephalon in fetal rats. In newborn rats, the density of nNOS immunoreactive cells was the highest at P0 in the cerebral cortex and striatum, and reduced sequentially at P5 and