Irradiation combined with Sorafenib in hepatoma cells the role of the different time.docVIP

 PAGE \* MERGEFORMAT 19 Irradiation combined with Sorafenib in hepatoma cells the role of the different time Of: Lee Qiaoqiao, Liumeng Zhong, Wang Li, Li Jinqing [Abstract] [aim] observed in human hepatoma cells irradiation combined with Sorafenib on cell growth, and to explore its effect. [Methods] human liver cancer SMMC-7721 and BEL-7402 cells were divided into three radiation group (IR), 30 min before radiation Sorafenib combined group (IR + S-pre) and 24 h after radiation combined Sorafenib group (IR + S-post). cell colony formation assay colony formation rate after irradiation, cell survival curves drawn radiosensitization ratio was calculated; cell proliferation assay after 6 Gy irradiation cell proliferation 1,2,3,4,5,6 d; immunofluorescence staining DNA damage after irradiation, the proportion of positive cells; flow cytometry apoptosis after radiation ratio. [Results] The results showed that colony formation, IR + S-pre increase in colony formation after radiation, radiosensitization than in the SMMC-7721 and BEL-7402 cells were 0.78 and 0.88; and IR + S-post reduction of colony formation after radiation, radiosensitization than in the SMMC-7721 and BEL-7402 cells were 1.43 and 1.23. cell proliferation inhibition experiments showed that IR + S-pre and post-IR radiation inhibition of cell proliferation rate similar, and IR + S-post after radiation significantly increased cell proliferation. Sorafenib in SMMC-7721 and BEL-7402 induced apoptosis in both. IR + S-pre in the cells 24 h after radiation was significantly increased [IR vs IR + S-pre: (6.1 + - 1.0)% vs (18.3 + - 2.0)% (SMMC-7721), (8.2 + - 2.1)% vs (17.0 + - 2.4)% (BEL-7402 ), P lt;0.001]. IR + S-post in the cells 48 h after radiation was significantly increased, [IR vs IR + S-post into (6.9 + - 2.0)% vs (15.9 + - 1.8)% ( SMCC-7721), (8.0 + - 1.5)% vs (14.2 + - 2.5)% (BEL-7402), P lt;0.05]. radiotherapy after 30 min, IR + S-pre and IR positive cells in the DNA damage no significant differ