Left and right Pill Pill autoimmune encephalomyelitis in rats on lymphocyte subsets in the central nervous system immunohistochemical expression of_0.docVIP

 PAGE \* MERGEFORMAT 16 Left and right Pill Pill autoimmune encephalomyelitis in rats on lymphocyte subsets in the central nervous system immunohistochemical expression of Study: Fan Yongping, Song Lijun, Ye Ming, Zhou Li, Gong ocean, Wang Lei, Liu Yan [Abstract] Objective To observe the left and right Pill Pill experimental autoimmune encephalomyelitis (EAE) model of rat brain and spinal cord tissue lymphocyte subsets immunohistochemical expression, and Enriching Kidney Treatment of kidney yang mechanism of EAE. Methods of myelin basic protein with complete Freund’s adjuvant, 1:1 by volume and in Lewis rats made double antigen footpad injection after establishment of EAE model .120 only Lewis rats were randomly divided into normal group, model group, hormone group, the left group and right return Pill pill group, n = 24, the normal group and model group were given normal saline, 3 mL / (only d) ; hormone group given saline after immunization, 3 mL / (only d), after the onset of suspension fed to prednisone, 5 mg / (kg · d); Zuoguiwan group given after immunization Zuoguiwan suspension, 2 g / (kg · d); group immunized Youguiwan given Youguiwan suspension, 3 g / (kg · d). until death. were the acute phase (15 after immunization d) and remission (after immunization, 27 d) were randomly selected rats in each group, and the brain and spinal cord tissue sections were CD4 +, CD8 +, CD3 +, CD19 + immunohistochemical staining. Results after modeling, the 15th day and 27 days, model in brain and spinal cord tissue CD4 + immunohistochemical expression than the normal group increased (P lt;0.01), CD8 + was decreased (P lt;0.05), CD4 + / CD8 + ratio increased (P lt;0.01); with model group, the left Gui Wan Group, Gui Wan Group, hormone group CD4 + immunohistochemical expression were lower (P lt;0.01), and in after modeling, 27, Zuoguiwan group and Youguiwan spinal cord tissue CD4 + expression compared with steroid group was significantly lower (P lt;0.01); after mode