Low-oxygen environment of arsenic trioxide on human lung cancer cell proliferation and apoptosis.docVIP

 PAGE \* MERGEFORMAT 7 Low-oxygen environment of arsenic trioxide on human lung cancer cell proliferation and apoptosis Author: Shen Jie, Qu Song-Ping, Xiu-Qing Yu, Li Bing [Abstract] Objective: To observe the normoxic and hypoxic conditions with different concentrations of arsenic trioxide (arsenic trioxide, As2O3) on human lung adenocarcinoma A549 cell proliferation and apoptosis. Methods: normoxia (21% O2) and hypoxia (5% O2) environment on human lung adenocarcinoma A549 cells were cultured in vitro, using 0,1,2,4 μ mol / L As2O3 role of A549 cells, respectively, 12,24 and 48 h, methyl thiazolyl tetrazolium (methyl thiazolyl tetrazolium, MTT) assay A549 cell proliferation rate; Annexin Ⅴ (Annexin Ⅴ ) / PI (propidium iodide, PI) to detect double-labeled cells, wither death. Results: Under normoxia and hypoxia, 1,2,4 μ mol / L As2O3 could significantly inhibit A549 cell proliferation, promote cell apoptosis, and will increase with the concentration of As2O3 and the role of the extension of time to enhance; the role of the in both environments was no significant difference. CONCLUSION: As2O3 could inhibit A549 cell proliferation and promote apoptosis; in the hypoxic environment and under normal oxygen environment, A549 cells have the same cytotoxicity. [Keywords:] Arsenic compounds; hypoxia; apoptosis; cell proliferation; lung cancer Objective: To observe the effects of different concentrations of arsenic trioxide (As2O3) on apoptosis and proliferation of human lung cancer cell line A549 in vitro under hypoxia and normoxia. Methods: A549 cells were treated with 0, 1, 2, 4 μ mol / L As2O3 for 12, 24 and 48 h under hypoxia (5% O2) and normoxia (21% O2). The proliferative inhibition rate of A549 cells was measured with methyl thiazolyl tetrazolium assay, and the apoptotic rate of A549 cells was detected by Annexin Ⅴ / propidium iodide (PI) double staining. Results: Under normoxia and hypoxia, 1, 2, 4 μ mol / L As2O3 could significantly inhibit the prolifer