Microwave-assisted extraction - high performance liquid chromatography of 6 Vegetables - BA.docVIP

 PAGE \* MERGEFORMAT 5 Microwave-assisted extraction - high performance liquid chromatography of 6 Vegetables - BA Study: Liu Haiyan Zhang Jie Lin-Lin Wang Yan Wang Yinghua Li Shan Chen [Keywords:] extraction high performance liquid chromatography Vegetables 6 - benzyladenine 1 Introduction 6 - benzyladenine (N6-Benzylaminopurine ,6-BA as a plant growth hormone, not only widely used in plant cultivation areas, can also be used for vegetables, fruits, Preservation, commercial vegetables and fruits may be a small amount of residue. Human consumption 6-BA will stimulate excessive skin and mucous membranes, damage the esophagus and gastric mucosa, nausea, vomiting and, therefore, the provisions of GB2760-1996 6-BA in food highest residue was 0.2 mg / kg. has not yet been established in food in China 6 - BA residue limits of detection. microwave assisted extraction (MAE to extract a short time, with less extraction solvent, extraction yields, etc. are widely used. In this study, MAE extraction, HPLC separation to detect vegetable residues 6-BA volume. This method has low detection limits, high recovery, extraction time, etc., for real samples with satisfactory results. 2 Experimental part 2.1 Instruments and reagents Agilent-1100 liquid chromatograph (Agilent, USA, with a UV detector, KQ-200VOE ultrasonic extractor (China Kunshan Ultrasonic Instrument Co., Ltd., SC-3610 centrifuge (HKUST innovative stock limited good company branch), WR-E-type microwave sample preparation system (Beijing United Brinckerhoff, R201 rotary evaporator (British Yu Gongyi Instrument Co., Ltd. for China .6 - BA standards (contentgt; = 99.0 %, Fluka Company, acetonitrile and methanol (HPLC grade, Fisher U.S. companies, glacial acetic acid (HPLC grade, acidified methanol: 100 mL of methanol - water (7:3, V / V acetic acid added to 50 μL, test water for the Wahaha pure water. 2.2 HPLC determination condition Venusil XBP-C18 column (250 mm × 4.6 mm, 5 μm, the United States