Morphological typing difficulties in 24 cases of leukemia immunophenotyping.doc

 PAGE \* MERGEFORMAT 6 Morphological typing difficulties in 24 cases of leukemia immunophenotyping Author: Sun Ying-hui, Ma early Lin Di, Yu-Hui Ying, when Bao Fu, Lu Yan [Keywords:] leukemia; Immunophenotyping; flow cytometry Immunophenotyping in 24 patients with leukemia in comparison with morphotyping [Abstract] AIM: To study the immunophenotyping with flow cytometry rapidly and efficiently in 24 patients with acute or chronic leukemia. METHODS: Immunophenotyping was performed by doublelabeling with different antibodies conjugated with FITC and PE and analyzed with multiparameter flow cytometry for 24 patients with acute or chronic leukemia, which were difficult to be diagnosed clinically by morphology. RESULTS: ① Nine patients clinically diagnosed as chronic lymphocytic leukemia were immunophenoyped as B lymphocytic leukemia by FCM; ② Five patients clinically diagnosed as acute lymphocytic leukemia (L2) were immunophenotyped as different leukemia groups by FCM, including 1 patient with B lymphocytic leukemia, 2 with T lymphocytic leukemia (one of them with erythrocytic abnormity), 1 with undifferentiated leukemia, and 1 with lymphocytic leukemia expressing both B and T cell antigens; ③ Five patients clinically diagnosed as acute nonlymphocytic leukemia were immunophenotyped by FCM as 2 patients with myeloid leukemia, 3 patients with erythroleukemia (M6); ④ One patient clinically diagnosed as MDS with myelofibrosis was immunophenotyped as M6 by FCM; ⑤ Among 4 patients who were suspected as both myeloid and lymphoidlineage phenotype, only 2 were diagnosed as double myeloid and lymphoid markers positive phenotype. The coincidence between immunophenotype ane FAB phenotype was 79.2%. CONCLUSION: Immunophenotyping by FCM may retrieve the faults of FAB phenotyping, providing important evidences for clinical diagnosis and guiding treatment individually . [Keywords] leukemia; immunophenotype; flow cytometry [Abstract] Objective: Using flow cytometry in 24