Mycobacterium tuberculosis (H37Rv) secreted protein bioinformatics prediction method.doc

 PAGE \* MERGEFORMAT 13 Mycobacterium tuberculosis (H37Rv) secreted protein bioinformatics prediction method [Keywords:] Mycobacterium tuberculosis Bioinformatics prediction strategy for Mycobacterium tuberculosis (H37Rv) secreted proteins [Abstract] AIM: To establish a prediction strategy for Mycobacterium tuberculosis (H37Rv) secreted proteins to pave the way for further research. METHODS: The whole protome of H37Rv was scanned by SignalP and TMHMM. The protein date analysis system based on Visual FoxPro was established to process the output of SignalP and TMHMM and identify the secreted proteins. The sequences of the secreted proteins were aligned by BLASTp. RESULTS: One hundred and seventynine secreted proteins were identified, where 12 of them were found to be unique in H37Rv. CONCLUSION : Bioinformatics approaches can be used as an assistant tool in secreted protein research. [Keywords] Mycobacterium tuberculosis; secreted protein; signal peptide; bioinformatics [Abstract] Objective: To establish a Mycobacterium tuberculosis (H37Rv) secreted protein prediction method, provide a reference for follow-up study. Methods: SignalP and TMHMM two software Mycobacterium tuberculosis proteome scans, based on Visual FoxPro to build ‘protein data analysis and processing system’ of the original scan data analysis and processing to identify secreted proteins, and then completed by the BLASTp similarity comparison. Results: The predicted out of 179 kinds of secreted proteins, of which 12 species are unique to H37Rv. Conclusion: The bioinformatics method can be used as a research secreted proteins support tool used to guide experiments. [Keywords:] Mycobacterium tuberculosis; secreted protein; signal peptide; bio-informatics 0 Introduction Mycobacterium tuberculosis (Mycobacterium tuberculosis, MTB) of the secreted protein not only in guinea pigs can lead to delayed-type hypersensitivity, in TB patients can also be induced in vivo production of antibodies [1], th