Nanhu gel anti-inflammatory analgesic effect and its mechanism.doc

 PAGE \* MERGEFORMAT 5 Nanhu gel anti-inflammatory analgesic effect and its mechanism Author: Kong Xiangyan, Xiao Chang-Hong, Wu Qifu, Yi Shi-hong [Keywords:] Nanhu gel Antiinflammatory and analgesic effects of Nanhu Gelata and its mechanism [Abstract] AIM: To estimate the antiinflammatory and analgesic effects of Nanhu Gelata and to study its mechanism. METHODS: Xyleneinduced mouse ear edema models and carrageenaninduced rat paw edema models were used to study the antiinflammatory effects. Hotplate test in mice was used to study the analgesic effects. The contents of IL1 and TNFα in the carrageenaninduced rat paw edema tissues were measured using rat IL1β ELISA and TNFα ELISA kits to explore the mechanism of its antiinflammatory and analgesic effects. RESULTS: Nanhu Gelata significantly suppressed xyleneinduced mouse ear edema and carrageenaninduced rat paw edema. The pain threshold of hotplate test mice was remarkably improved by Nanhu Gelata. The content of IL1 and TNFα in the inflammatory tissue extracts significantly decreased. CONCLUSION: Nanhu Gelata has some significant antiinflammatory effects on acute inflammatory reactions. Nanhu Gelata significantly relieves pain reactions induced by heat. The mechanism of antiinflammatory and analgesic effects of Nanhu Gelata may be related to its effect of decreasing the IL1 and TNFα content in inflammatory tissues. [Keywords] Nanhu Gelata; antiinflammatory effects; analgesic effects; mechanism [Abstract] Objective: To evaluate the Nanhu analgesic effects of anti-inflammatory gel to reveal the mechanism of its anti-inflammatory analgesic. Methods: xylene-induced mouse ear swelling and carrageenan-induced rat paw feet swelling model, study Nanhu gel anti-inflammatory effect; hot-plate gel was observed by Southern Tigers analgesic effect. IL1β ELISA kit in rats and rat TNFα ELISA detection kit carrageenan-induced inflammatory rat paw swelling of the tissues of IL1 and TNFα levels to understand the Sout