Nicotine on macrophage expression of liver X receptorand cholesterol efflux.doc

 PAGE \* MERGEFORMAT 13 Nicotine on macrophage expression of liver X receptorand cholesterol efflux Study: Money Chung Chieh Tseng Chiu-tong, Qiu Ling, Xiao Chuan real [Abstract] Objective To study the effect of nicotine on the liver macrophage receptor X @ (LXR @) and its downstream gene expression and cholesterol efflux, of nicotine on the role of LXR signaling. Methods Human peripheral blood mononuclear cells and transformed into macrophages. in the role of nicotine observed aopA macrophage-mediated cholesterol efflux, and LXR mRNA expression of several genes downstream. Results significant effect of nicotine in the number of cells involved in macrophage cholesterol metabolism and inflammatory gene expression, while reducing aopA mediated cholesterol efflux. Conclusions Nicotine macrophage LXR signaling pathway, promoting foam cell formation, so that the development of atherosclerosis. [Keywords:] nicotine, cholesterol efflux, macrophages, liver X receptor ABSTRACT: Objective To investigate the characteristics of liver X receptor alfa (LXR @) and its target gene expression as well as cholesterol efflux from human macrophages under nicotine. Methods Human monocyte derived macrophages were collected. Before apoA I mediated human monocyte derived macrophage cholesterol efflux , and mRNA expression of LXR @ and some of its target genes being detected, the macrophages were induced with or without nicotine. Results Preincubation of human monocyte derived macrophages with nicotine, cholesterol efflux was suppressed to apolipoprotein AI. Nicotine also inhibited LXR @ and some of its target genes mRNA expression involved cholesterol metabolism, and facilitated expression of some inflammatory genes. Conclusion Nicotine can regulate foam cell formation by inhibiting LXR pathway and produce atherogenesis. KEY WORDS: nicotine; cholesterol efflux; macrophage; liver X receptor Early atherosclerotic lesions of cholesterol-rich vascular subendothelial acc