Of age on rat adipose stem cell osteogenic differentiation of.doc

 PAGE \* MERGEFORMAT 13 Of age on rat adipose stem cell osteogenic differentiation of Of: Yun Tao Qin Shujian Dan Pei Chao Ren Quanta [Abstract] Objective To study age on adipose derived stem cell osteogenic differentiation. Methods by density gradient centrifugation of different ages ADSCs were cultured and passaged, observed cell growth, cell culture to passage 3 were induced to differentiate, all ages osteogenic cells were detected after induction of differentiation. to observe the morphological changes, apoptosis rate, alkaline phosphatase activity and calcium ion concentration determination. ADSCs in vitro results showed a spindle, induced conditions, cells in each age group shape into a cube from the spindle, polygonal, after testing, increase with age in the apoptosis rate is increasing, and young cells than the older group of high alkaline phosphatase activity, calcium ion concentration decreased with age. Conclusion The ability of ADSCs into osteoblasts decreases with increasing age. [Keywords:] age of tissue engineering stem cells into osteoblasts The choice of seed cells for bone tissue engineering is the foundation and one of the keys. Mesenchymal stem cell (Bone marrow stromal stem cells. BMSCs seed cells for bone tissue engineering has been widely used. But the limited amount of bone marrow collection, a large number of extraction will increase the body injury and have some complications [1], so not conducive to the cultivation and application of large-scale clinical .2001 Zuk et al [2] obtained by liposuction of fat stem cells (Adipose-derived stem cells, ADSCs). ADSCs have a rich source of and easy access to [3], autologous transplantation can avoid rejection, and has differentiated into a variety of mesoderm derived cells [4] and cross-mesoderm differentiation [5], etc., a large number of studies confirm ADSCs in vitro to stabilize growth and passage, and with osteogenic differentiation capacity [6,7], but different ages ADSCs diffe