On poly human pregnancy extravillous trophoblast regulation of TLR3 expression.docVIP

 PAGE \* MERGEFORMAT 16 On poly human pregnancy extravillous trophoblast regulation of TLR3 expression [Abstract] Objective To study Toll-like receptor in vitro 3 (TLR3) in human first trimester extravillous trophoblast cell line TEV-1 expression and poly PolyI: C for TLR3 mRNA and protein regulation. TEV cultured in vitro -1 cells; time and with different concentrations of poly stimulate TEV-1 cells RT-PCR and flow cytometry TLR3 mRNA and protein expression; immunohistochemistry SP staining the expression of TLR3 protein location. Results (1) PolyI: C stimulation can increase TEV-1 cell line TLR3 mRNA expression (P lt;0.01), and with the stimulation time and dose were positively correlated. (2) PolyI: C stimulation can increase TEV-1 cell line TLR3 protein expression of mean fluorescence intensity (P lt;0.01); but the fluorescence-positive cells stimulated 12h with the stimulation the difference was not statistically significant (Pgt; 0.05); stimulate 24h, 48h of fluorescent positive cells and the stimulation time and dose were positively related (24h P lt;0.05; 48h P lt;0.01). (3) Immunocytochemistry showed TLR3 localized in the TEV-1 cells cytoplasm. Conclusions TEV-1 cell line the mRNA and TLR3 protein expression and Poly (I: C) stimulation time and dose were positively correlated; TLR3 localized in the TEV-1 cells cytoplasm, and nucleus and plasma membrane expression was not observed. Tip extravillous trophoblast in early human pregnancy through the cytoplasm of TLR3 may be involved in early pregnancy - maternal interface of the immune response. [Keywords:] TLR3; Poly (I: C); TEV-1 cell line; flow cytometry; mean fluorescence intensity; fluorescent positive cells; Immunocytochemistry [Abstract] Objective To investigate the regulatory effect of PolyI: C on the expression of TLR3 mRNA and protein in the human extravillous trophoblast cell line (TEV-1), and whether the regulatory effect depends on PolyI: C’s dose and time.Methods Semi -quantitative