p27mt gene in colorectal cancer Lovo cell adhesion Effect.docVIP

 PAGE \* MERGEFORMAT 12 p27mt gene in colorectal cancer Lovo cell adhesion Effect 【Abstract】 Objective To study the mutant p27 gene (p27mt) in colorectal cancer Lovo cell adhesion, and to explore p27mt gene in colorectal cancer Lovo cell transfer in the mechanism of action. Methods Ad  p27mt transfected colorectal cancer Lovo cells, using Western blot detection of p27mt expression in Lovo. Were used cell counting, MTT assay p27mt gene on colorectal cancer Lovo cell adhesion homogeneous, heterogeneous adhesion effects. The results of Ad  p27mt transfected Lovo cells, homogeneous adhesion is higher than Ad  LacZ group and the control group, heterogeneous adhesion below the Ad  LacZ group and the control group. Conclusion increase p27mt gene expression, can colorectal cancer increased adhesion between the homogeneous, heterogeneous adhesion reduction, therefore, p27mt genes inhibit the transfer function of colorectal cancer, its mechanism is to change the cell matrix and cell adhesion between the ability to. Keywords: p27mt gene; colorectal cancer Lovo cell; homogeneous adhesion; heterogeneity of adhesion Fund Project: Science and Technology Bureau of Shiyan City funded projects (2005ZD036) The Adhesion Effect of Colorectal Carcinoma Lovo cells by Human Mutant p27mt Gene SONG Shi  mao1, CHEN Jun2 1.Department of Oncology, Affiliated Taihe’s Hospital, Affiliated People’s Hospital, Yunyang Medical College, Shiyan 442000, China; 2.Department of Gastroenterology Corresponding author: CHEN Jun, E  mail: sycj@sycatv.netAbstract: Objective To study the effect of p27mt transfected into human colorectal carcinoma Lovo cells, and to explore the mechanism of p27mt gene on the metastases of colorectal carcinoma Lovo cells.Methods The expression of p27mt on the p27mt  transfected Lovo cells was determined p27mt protein expression with Western Blot. The effect of p27mt gene on the homogeneity adhesion of human colorectal carci