Over-expression of FHIT gene in human gastric cancer cell line MKN-45 proliferation and adhesion_0.docVIP

 PAGE \* MERGEFORMAT 14 Over-expression of FHIT gene in human gastric cancer cell line MKN-45 proliferation and adhesion Of: Huang Zhao-quan, Liu Fei, Pei-Lin Huang, Li Nan, Xu Jiajia, Wu Peng [Abstract] Objective To investigate the overexpression of FHIT gene in human gastric cancer cell line MKN-45 proliferation and adhesion. Methods will include some exogenous FHIT gene eukaryotic expression plasmid pcDNA3.1-FHIT gene transfection of FHIT mRNA negative Expression of human gastric cell line MKN-45, respectively MTT, colony formation assay, adhesion assay cell proliferation after transfection and colony forming ability and adhesion. Results FHIT gene over-expression can reduce the MKN-45 cell proliferation and colony formation (P lt;0.05, but on the MKN-45 cells and endothelial cells had no effect on adhesion. Conclusions FHIT gene over-expression inhibits human gastric cancer MKN-45 cells proliferation and colony formation, for MKN-45 cell adhesion to endothelial cells had no significant effect. [Keywords:] FHIT cloning of gastric tumor cell proliferation cell adhesion molecule gene Abstract: Objective To evaluate the effects of FHIT gene over-expression on human gastric cancer cell line MKN-45 proliferation and adhesion. Methods Human xenogenous eukaryotic expression plasmid pcDNA3.1-FHIT were transfected to mRNA-negative human gastric cell line MKN-45 . A MTT assay, plate clony-forming test and adhesion test were performed to assess the MKN-45 cells ‘proliferative activity, clony-forming capability and adhesive ability before and after transfection. Results FHIT over-expression can reduce MKN-45 cells’ proliferation and clony-forming capability (P lt;0.05), but it was ineffectiveness on MKN-45 cells and endothelial cells adhesion. Conclusion FHIT over-expression can inhibit the proliferation and clony-forming ability of human gastric cell line MKN-45, it dose not work on MKN-45 cells and endothelial cells adhesive ability. Keywords:: FHIT; ga