PDMS chip enzyme reactor Glucose free.doc

 PAGE \* MERGEFORMAT 14 PDMS chip enzyme reactor Glucose free Of: Zhong Haiyan Zhou Jie Yu Xiaodong CHEN Hong-Yuan [Abstract] Micro gel electrophoresis analysis of intermediary (EMMA technology, build a polydimethylsiloxane (PDMS chip enzyme reactor free, online detection of glucose (Glu, in the cross-channel chip, using home-made carbon fiber microelectrodes Glucose oxidase (GOD catalyzed oxidation of Glu-generated H2O2, and detection potential, GOD concentration, GOD injection time, separation voltage and other parameters were optimized to determine the free enzyme reactor linear range and detection limit was investigated The reproducibility and stability. The results show that the production of free enzyme reactor convenient, simple, reproducible, Glu concentration in the 0.1 ~ 20 mmol / L a good linear relationship between (r = 0.997, The detection limit was 19.8 μmol / L (S / N = 3. [Keywords:] free enzyme reactor, glucose, electrophoretic analysis of intermediate micro, polydimethylsiloxane chip The Key Lab of Analytical Chemistry for Life Science, Ministry of Education, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093) Abstract Microfluidic enzyme based reactor could be used to detect the biomolecules with high sensitivity and selectivity. Based on the electrophoretic mediated microanalysis (EMMA) method, a new kind of free enzyme based poly (dimethylsiloxane) (PDMS) microchip was developed to detect glucose (Glu). On line catalysis reaction of Glu by glucose oxidase (GOD) was carried out on the chip. The product H2O2 was detected using single carbon fibre cylindrical electrode. Factors influencing the separation and detection, such as detection potential, GOD concentration, GOD injection time and separation voltage, were investigated and optimized. Results showed that the peak current had a good linear relationship with Glu concentration in the range of 0.1-20 mmol / L (R = 0.997). The detection limit of Glu w