Pei Fang demolition side of this regulation asthmatic rats the role of endogenous glucocorticoid pathway Experimental Study.doc

 PAGE \* MERGEFORMAT 9 Pei Fang demolition side of this regulation asthmatic rats the role of endogenous glucocorticoid pathway Experimental Study 【Abstract】 Objective To study the pui this side of asthma endogenous glucocorticoid (GC) The role of the pathway in vitro. Methods OVA (ovalbumin) sensitized to stimulate rat model of chronic asthma, to Aspen, America capsules as the control drug, by radioimmunoassay of serum CORT (corticosterone), plasma ACTH (adrenocorticotropic hormone) and the hypothalamic CRH (corticotropin-releasing hormone) levels and immunohistochemical staining in lung tissue GCR (glucocorticoid receptor) expression to explore the Pui right side of this square and its demolition of part of the role of rat asthma model of the mechanism. The results of model control group than in the normal control group, CORT and ACTH levels were significantly reduced, GCR protein expression is significantly reduced, CRH showed no significant change; Pei side of the side group and split the whole side group Ⅱ improve CORT, ACTH, GCR’s influence is more as significant. Conclusion The role of regulation of endogenous GC in this way may be pui side one of the mechanisms of anti-asthmatic effects. Keywords: Pei split side of the square of endogenous glucocorticoids in asthmatic rats AbstractObjectiveTo study the adjustment of Peibenfang on the functional route of endogenous GC in asthma rats. MethodsAsthma rats model was established by OVA through sensitizing and challenging. Asimei Capsule was used as positive control medicine. By investigating CORT in blood serum, ACTH in blood plasma, CRH in hypothalamus and GCR in the lung issue, the effects of Peibenfang on asthma were observed.ResultsThe levels of CORT, ACTH and GCR in model group were lower than those in normal group; in Peibenfang group and Peibenfang compatibility group (part Ⅱ), these indexes increased remarkably. ConclusionOne of the mechanisms of Peibenfang against asthma is to