Peng fingerprint of medicinal Curcuma Research.doc

 PAGE \* MERGEFORMAT 12 Peng fingerprint of medicinal Curcuma Research Authors: Xin Zhou, Chen Huaguo, Zhao Chao, GONG See, Ming - [Abstract] Objective To establish the Punta Curcuma herbs volatile components by gas chromatography - mass spectrometry (GC-MS) fingerprinting, in order to Punta Ezhu the quality control of medicinal herbs provide a reference. Methods steam distillation Punta Zedoary volatile oil, GC-MS in conjunction with the technology used to fingerprint their determination and to use ‘similarity of chromatographic fingerprint evaluation software’ on the similarity between the different samples calculated. The results initially established Punta Curcuma Essential Oil GC-MS fingerprints, calibration of a total of 20 peaks, each batch of medicinal herbs have these characteristics, but the characteristic peak there is a certain difference in the relative content. Conclusion This method has good reproducibility and precision, combined with GC-MS also has a qualitative advantage of multi-component, the establishment of Punta Curcuma Essential Oil GC-MS fingerprint for quality control provides a scientific basis. [Keywords:] Peng Volatile oil Curcuma GC - MS fingerprint Abstract: ObjectiveTo establish a method of fingerprint analysis on volatile oil of Curcuma phaeocaulis Valeton by GC-MS. MethodsThe essential oil of Curcuma phaeocaulis Valeton growing in Shuangliu city of Sichuan province was extracted by steam distillation and the chromatographic fingerprint was characterized by GC-MS . The GC-MS analysis was carried out in a Hewlett Packard model 6890 gas chromatograph coupled with a Hewlett Packard model 5973 mass detector. A Hewlett Packard HP-5 (5% phenyl methyl siloxane) fused silica capillary column (30 m * 0.25 mm ID, 0.25μ m film thickness) was used for the separation. ResultsWe established Mutual fingerprint of the essential oil of Curcuma phaeocaulis Valeton by using the similarity calculation software of Chinese herbal fingerprint desi