Poria and other 9 kinds of traditional Chinese medicine screening of estrogenic activity of plant experimental study.doc

 PAGE \* MERGEFORMAT 11 Poria and other 9 kinds of traditional Chinese medicine screening of estrogenic activity of plant experimental study Author: Pali Naka Si da Abliz hot wood Shengping Cong Yuanyuan, Hattori Yukio [Abstract] Objective of several traditional Chinese medicine made of plant estrogen activity screening. Methods of human breast cancer cell proliferation law and the yeast estrogen screening experiment carried out on nine kinds of traditional Chinese medicine estrogenic activity of plant research. Succeeded in establishing the E-SCREEN law and the Yeast Estrogen Screen experimental method, this can be detected in two ways 1 * 10-13 mol / L of estradiol. In both in vitro screening of estrogen activity in the experimental conditions, angelica, Alismataceae, Chuanxiong, Cortex Moutan, Guizhi have estrogenic activity; and Poria, peach kernel had no estrogen-like activity. Conclusion E-SCREEN method high sensitivity and a fast, simple and the advantages of a large number of compounds applied to primaries. [Keywords:] human breast cancer cell proliferation France yeast estrogen screening of estrogenic activity experiments Abstract: ObjectiveTo evaluate the estrogenic activity of several kinds of Chinese herbal medicines. MethodsE-Screen and Yeast Estrogen Screen technology were used to study the estrogenic activity of nine Chinese herbal medicines. ResultsThe two methods detected estradiol at very low concentration as 1 * 10 -- 13mol / L. It was found that Angelica acutiloba Kitagawa var.sugiyamae Hikono., Alisma orientale Juzepczuk., Cnidium officinale Makino., Paeonia suffruticosa Andr., Cinnamomum cassia Blume. had estrogenic effects, while Poria cocos wolf.and Prnus persica Batsch. lacked estrogenic effects at this assay.ConclusionThe E-SCREEN test is sensitive, rapid and easy to perform.It ‘s suitable for large-scale screening for estrogenicity of environmental chemicals. Keywords:: E-SCREEN; Yeast Estrogen Screen; Estrogenic activity Plan