Preparation of electrical integration of osteosarcoma fusion cells tumor vaccine Analysis.doc

 PAGE \* MERGEFORMAT 25 Preparation of electrical integration of osteosarcoma fusion cells tumor vaccine Analysis 【Abstract】 Objective of this study is to prepare osteosarcoma fusion cell vaccine and tested its in vitro biological properties. Methods Preparation of electrical integration UMR  106 cells and rat macrophage fusion cells, after magnetic sorting after cell fusion by flow cytometry DNA content and surface antigen expression. Detection of cell culture clone forming ability of fusion cells, and measured in vitro killing activity of CTL induction. The results of electrical integration and combination of magnetic separation technology, available to 95% of the fusion cell purity. Flow cytometry showed that fusion cells can express high MHC  Ⅰ, Ⅱ antigen. Its soft-agar colony forming ability decreased significantly (UMR  106,29.2 ± 7.2, fusion cells, 17.6 ± 5.1, P lt;0.05), while the ability to induce CTL cytotoxicity was significantly increased (100:1, when UMR  106 13.4 ± 3.2 , fusion cells, 42.9 ± 7.5, P lt;0.05). Conclusion osteosarcoma fusion cells can stabilize the growth of CTL cells in vivo induced a strong anti-tumor activity in animal models can be used to test their effect induced by anti-osteosarcoma. Keywords: Osteosarcoma cell fusion vaccine Characterization of Osteosarcoma Vaccine Cells Prepared by Electrical Cell Fusion Abstract: Objective Prepare the osteosarcoma vaccine by cell fusion and detect itsbiological properties.Methods The osteosarcoma vaccine was obtained by fusing UMR  106 and rat macrophages with electorporator.The DNA and surface antigen expression of fusion cells purified by magnetic cell sorting unit were assayed with flowcytometry.The colony assay of the cells and the analysis of CTL activity induced by fusion cells in vivo were also performed.Results The higher cell fusion efficiency can be obtained with electroporator and about 95% purity of fusion cells achieved by magnetic cells sorting un