Preparation of ginkgo phospholipid complex process optimization.doc

 PAGE \* MERGEFORMAT 16 Preparation of ginkgo phospholipid complex process optimization 【Abstract】 Objective To optimize the formation of Ginkgo biloba extract and phospholipids complex preparation process. Methods of Flavonoids of Ginkgo biloba extract compound rate of assessment criteria, using single-factor (solvent) and orthogonal experimental design study of various factors on the impact of recombination rate; and high-performance liquid chromatography in ginkgo phospholipid complex content of flavonol glycosides . The results determine the ginkgo phospholipid complex at room temperature, the optimal preparation conditions, namely, THF as the reaction solvent, gingko and phospholipids of the feed ratio of 1:2, reaction time 20 h. Conclusion at room temperature, ginkgo phospholipid complex formation by the solvent, reactant feed ratio, reaction time, a greater impact. 【Key Words】 Ginkgo biloba extract phospholipid complex orthogonal design preparation process AbstractObjectiveTo optimize the preparing condition of Ginkgo-phospholipid Complex.Methods The study was carried out through single factor experiment and orthogonal design with the complex yield of flavone as the guide. In addition, the content of flavone in Ginkgo-phospholipid Complex was also determined by HPLC.Results Under room temperature, the optimal preparing conditions of Ginkgo-phospholipid complex was as follows: the ratio of Ginkgo biloba extract (EBG) and phospholipid was 1:2, the solvent was THF and the reacted time was 20 h. The Ginkgo -phospholipid Complex prepared under the optimal conditions had the highest content of flavone. ConclusionUnder room temperature, solvent, reaction ration and reaction time have prominent effect on the preparation of Ginkgo-phospholipid Complex. Keywords:Ginkgo biloba extrat (EBG); Phospholipid complex; Orthogonal design; Preparation In recent years, the natural active ingredients phospholipid complex studies have rep