Pseudomonas aeruginosa integron class I-related genes analysis and positioning.docVIP

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Pseudomonas aeruginosa integron class I-related genes analysis and positioning.doc

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Pseudomonas aeruginosa integron class I-related genes analysis and positioning

Pseudomonas aeruginosa integron class I-related genes analysis and positioning Author: Yang Weiqing HUANG Wen-Xiang Jia Yin Chang-fu Liu Guixia [Abstract] Objective of four clinical strains of Pseudomonas aeruginosa carrying class I integron-related genes to parse and position to explore the class I integron-related genes in the formation of bacterial resistance to antibiotics and resistance genes spread in the role. Methods using PCR amplification, DNA sequencing, DNA sequence alignment approach to the four clinical strains of Pseudomonas aeruginosa carrying class I integron-related gene analysis, using bonding tests to locate that type of integration of sub-analysis. Results 4 Class I integrons in Pseudomonas aeruginosa contain resistance genes, the resistance gene in the corresponding drug-resistant strains play a role; bacteria in the plasmid integrons. Conclusion I type integron resistance gene in Pseudomonas aeruginosa resistance to the formation and spread of resistance genes play a role. [Keywords:] Pseudomonas aeruginosa; I type integron; resistance gene ABSTRACT Objective Drug resistance genes associated with class I integron and their allocation in Pseudomonas aeruginosa were analyzed. Methods PCR screening and DNA sequence were used to determine the drug resistance genes associated with class I integron in 4 clinical strains of P.aeruginosa. The resistance genes were located by conjugated transfer experiments and the conjugations were studied by PCR method. Results Four clinical strains of P.aeruginosa carried drug resistance genes associated with class I integron, which played the drug resistant role in the clinical isolates and were located in plasmid. Conclusion Drug resistance genes associated with class I integron played the drug resistance and class I integron carried by plasmid were widespread easily in clinical isolates of P.aeruginosa. KEY WORDS Pseudomonas aeruginosa; Class I integron; Drug resistance gene Integrons (integron, int) is a