pten gene recombinant adenovirus on ovarian cancer cell growth inhibition.doc

 PAGE \* MERGEFORMAT 9 pten gene recombinant adenovirus on ovarian cancer cell growth inhibition Author: Lin Guan-ping, Xiong, Li Shumei, Belinda Wong, ZHOU Ke-yuan [Abstract] Objective: To observe the pten gene carrying wild-type adenovirus (Ad  pten) in vitro transfection of highly metastatic ovarian carcinoma HO  8910PM cells the expression and to study ovarian cancer cells inhibited their proliferation, inducing apoptosis and inhibiting migration. Methods: Using Ad  Easy Construction of adenovirus vector system, Ad  pten, in vitro transfection of highly metastatic ovarian carcinoma HO  8910PM cells, fluorescence microscopy detection of Ad  pten transfection efficiency. Western blot pten in HO  8910PM cells; MTT experimental observation pten on cell growth; HE staining of exogenous pten gene expression on cell morphology; cell scratch pten experimental observation of the inhibition of cell migration; Hoechest33258 apoptosis staining pten gene HO  8910PM induced apoptosis. Results: Exogenous wild-type pten gene successfully by adenovirus-mediated transfer HO  8910PM cells, Western blotting detected a PTEN protein expression, when the multiplicity of infection MOI for 100, the in vitro transfection efficiency of up to 90% or more. pten significantly inhibited HO  8910PM cell proliferation, induce apoptosis and to inhibit their migration. Conclusion: The recombinant adenovirus is a highly efficient gene transfer system that can efficiently target gene pten transferred to the highly metastatic ovarian carcinoma HO  8910PM cells, pairs of cells to produce a strong growth inhibition and apoptosis inducing effect, and can inhibit their migration. [Keywords:] ovarian tumor; gene therapy; pten gene; adenovirus; apoptosis; migration inhibitory 0 Introduction Tumor suppressor gene pten, or No. 10 chromosome deletion with the tension of proteins homologous genes, p53 then follows with a variety of tumor occurrence and development of a clos