Purification of autologous peripheral blood CD34 + cell transplantation in patients with systemic lupus erythematosus blood CD4 + CD25 + T cells of.doc

 PAGE \* MERGEFORMAT 15 Purification of autologous peripheral blood CD34 + cell transplantation in patients with systemic lupus erythematosus blood CD4 + CD25 + T cells of [Keywords:] Hematopoietic Stem Cell Transplantation CD4 + CD25 + T cells in systemic lupus erythematosus ABSTRACT: Objective To study changes of the peripheral blood CD4 + CD25 + T cells in patients with systemic lupus erythematosus after autologous peripheral blood purified stem cells transplantation. Methods Totally 15 patients with systemic lupus erythematosus who had received autologous peripheral blood purified stem cells transplantation were recruited in this study. Flow cytometry was used to measure CD4 + CD25 + T cells counting before transplantation and at 3, 6, 12, 24 months after transplantation. Results Before transplantation, the percentage of CD4 + CD25 + T cells was decreased. At 3, 6, 12, 24 months after transplantation, the CD4 + CD25 + T cells counting increased markedly compared with that before transplantation (P lt;0.05). There was no significant difference in percentage of CD4 + CD25 + T cells in pairwise comparisons of 6, 12, 24 months after transplantation. CD4 + CD25 + T cells of relapsed SLE were markedly lower than those of unrelapsed SLE. Conclusion Autologous peripheral blood purified stem cells transplantation can effectively increase the level of CD4 + CD25 + T cells in patients with systemic lupus erythematosus. KEY WORDS: stem cell; transplantation; CD4 + CD25 + T cell; systemic lupus erythematosus In recent years, autologous peripheral blood stem cell transplantation for systemic lupus erythematosus (systemic lupus erythematosus, SLE) achieved satisfactory results, the exact mechanism is not fully understood. But what is certain is that the original cell clone autoimmune been thoroughly destroy and rebuild a normal immune cell system is successful hematopoietic stem cell transplantation for the treatment of autoimmune disease key. autoimmunity and