Purification scutellarin orthogonal experimental design optimization.doc

 PAGE \* MERGEFORMAT 8 Purification scutellarin orthogonal experimental design optimization Of: Yao Xu, Liu Yun United States, Luo Xing, Huang Jin, Zhao Juan Ni, Zheng Xing [Abstract] Objective To study the purification process, improve scutellarin the purity and yield. Methods orthogonal experimental design, choose the best conditions scutellarin purification by high performance liquid chromatography purified samples scutellarin by HPLC. Results Ethanol ethanol 120 ml, 60% ethanol dissolved residue, acidic pH = 1 is the best purification process. Conclusion The method is simple, simple, and can get very good purity and yield. [Keywords:] scutellarin; orthogonal design; purification; high performance liquid chromatography Light flower only contained in the lt;lt;Southern Yunnan Materia Medicagt;gt;, also known as fleabane, Erigeron kiosks are short, classified Asteraceae Erigeron plants. Erigeron is about two hundred or more, and can be short of medicine Erigeron only 3 booths , since 1974, is widely used as the pharmaceutical industry in Yunnan Province pharmaceutical ingredients (lt;lt;Yunnan Drug Standardsgt;gt;. Breviscapine 70 agents from the 20th century began to be used clinically for the treatment of hypertension, cerebral embolism, multiple neuritis, retinitis, and chronic cerebral vascular accident due to paralysis [1]. It has been reported in recent years, treatment of cardiovascular and cerebrovascular diseases as the main active ingredient scutellarin (also known as scutellarin [ 2 ~ 4]. breviscapine now commercially available, mostly with scutellarin about 90% of the crude product, its further purification process also is not reported in the literature. In this study, orthogonal experimental method to study its purification process, using purified by high performance liquid chromatography scutellarin content of the sample [5 ~ 7]. 1 Apparatus and reagents High performance liquid chromatography (Shimadzu Corporation LC2010A type