Quantitative detection of serum HBV markers application experience.docVIP

 PAGE \* MERGEFORMAT 6 Quantitative detection of serum HBV markers application experience [Abstract] Objective quantitative analysis of serum HBV markers in clinical application. Qualitative methods using clinical samples (ELISA method, quantitative analysis detected by ELISA. Results of quantitative testing is more sensitive than the qualitative detection more stable. [Keywords:] quantitative detection of hepatitis B markers Hepatitis B is one of the world’s leading infectious disease, and serum markers in the clinical detection of hepatitis B (commonly known as two pairs of semi-qualitative determination began in the mid-20th century, 80 of hepatitis B disease diagnosis, monitoring, efficacy played a certain active role, but with the development of clinical medicine and laboratory medicine, qualitative detection can not meet the needs of clinical and patient, two pairs of semi-quantitative detection of hepatitis B is imperative, can greatly make up for lack of qualitative detection ELISA, light the superiority of quantitative detection of a few points: 1 monitoring of HBV markers greatly improve the detection sensitivity Quantitative Detection of HbsAg sensitivity of up to 0.2ng/ml, and ELISA (ELISA detection of HbsAg sensitivity up to 2ng/ml, serum HbsAg that will be presented to 2ng/ml ELISA positive. The quantitative analysis was able to check as soon as possible the HbsAg, confirmed HbsAg infection, almost simultaneously with the presi detection, disease detection in acute hepatitis B significantly reduced the detection of HbsAg the “window period”, while the qualitative detection of early often HbsAg were negative. If the virus mutates, the expression of more low, conventional ELISA antigen can not be detected, and quantitative with high sensitivity, can be found and is likely to detect HbsAg and HbsAb, serum HbsAg content and disease-free patients HbsAg cells is inversely proportional relationship, and changes in the liver and proporti