Quantitative detection of total aflatoxin ELISA Development Kit.docVIP

 PAGE \* MERGEFORMAT 7 Quantitative detection of total aflatoxin ELISA Development Kit [Abstract] Objective To study and establish sensitive, specific and fast food in general against Aflatoxin ELISA detection methods, to form China’s independent intellectual property rights with the rapid quantitative detection kit. Methods of aflatoxin in the production of monoclonal antibodies against the total, based on the use of indirect competitive ELISA method, developed a total aflatoxins in food rapid detection kits, and kits of the technical parameters were determined. The results of the kit standard on aflatoxin BG The minimum detectable concentration of 0  26ng/ml, the standard curve linear range of 0  26 ~ 20  00ng/ml, the linear equation y =- 0  4463x 0  3532 (R2 = 0  9915); of aflatoxin B G50% inhibitory concentration of 2  08ng/ml; Kit coefficient of variation of Article 4  18%, coefficient of variation of 5  21%, antibody affinity constant of 1  52 * 10-10mol / L; of corn plus three standard level of the average recoveries were 98  63%, 94  56% and 112  05%; of peanuts plus three standard levels of The average recoveries were 88  66%, 87  50% and 85  60%. Kit 37 ℃ can be placed more than 96h; Kit for Aflatoxin B1, B2, G1, G2 of the cross-reactivity rates were 100%, 57  5%, 104% and 19%, with no cross-reactivity to other fungal toxins. Conclusion of the ELIS kit developed can quantitatively detect the total aflatoxin in food. [Keywords:] total aflatoxin Aflatoxin (Aflatoxin) is the aflatoxin (XspeEillusflavus) and parasitic Aspergillus (A  parasiticus) and other similar structure produced by a group of secondary metabolites. 〔 1〕 Gabal, etc., according to reports, 40% of the cultures of Aspergillus flavus strains can simultaneously detect aflatoxins B1, B2, G1, G2 (AFB1, AFB2, AFG1, AFG2). The same time, the toxicity of aflatoxin have a synergistic effect, so more than 90% of the countries have established the total afla