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Qi times vitro herpes simplex virus type Ⅱ Experimental Analysis
PAGE \* MERGEFORMAT 11
Qi times vitro herpes simplex virus type Ⅱ Experimental Analysis
[Abstract] Objective To study the in vitro anti Ⅱ Qi times herpes simplex virus (HSV-Ⅱ) mechanism of action. Methods using in vitro cell culture techniques, the use of micro cytopathic effect inhibition method to study medicine each concentration group inhibit viral proliferation, directly Anti-Virus, the virus titer and viral suppression index for the index. Results ① inhibit viral proliferation experiments: Qi times 1.63,0.81,0.41 mg / mL concentrations of the virus titer and virus control group, the difference was statistically significant (P lt;0.01), suppressing the virus indexgt; 2, ② direct anti virus test: Qi times 1.63 mg/mL-8 h group, 1.63 mg/mL-24 h group, 0.81 mg/mL- 24 h group, the virus drops degree with the virus control group, the difference was statistically significant (P lt;0.05), other groups of virus titer and virus control group, no significant difference (Pgt; 0.05). Conclusion Qi times with anti-type Ⅱ herpes simplex virus.
[Keywords:] cervical erosion, Ⅱ-type herpes simplex virus, Qi times, virus titers, inhibition of virus index
Keywords:: cervical erosion, herps simplex virus-Ⅱ, Liq Qibei, virus titer; virus inhibition index
Chronic cervicitis, cervical erosion is a major pathological changes in obstetrics and gynecology common disease, the cancer rate is high. Qi times the powder film in the clinical formulations for the treatment of cervical erosion [1], the clinical observation and effective, [2]. We in the clinical study, based on the theory for the cause of the virus, using in vitro cell culture techniques, the use of micro cytopathic effect inhibition method [3] Qi times were anti-herpes simplex virus type Ⅱ (HSV - Ⅱ) in rats.
1 Materials
1.1 virus HSV-Ⅱ virus from Wuhan Institute of Virology, University College of Medicine gifts.
1.2 cells
Vero cells the virus by the Institute of Medicine, Wuhan University, and the
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