Radiation resistance of nasopharyngeal carcinoma cell line and differentially expressed genes.doc

 PAGE \* MERGEFORMAT 18 Radiation resistance of nasopharyngeal carcinoma cell line and differentially expressed genes Of: Wang Yali, Wang Xijing, Wang Zhongwei, Kim Ying Ying, Li [Abstract] Objective To screen the same source with different radiosensitivity differential gene expression in nasopharyngeal carcinoma cells, the mechanism of resistance to radiotherapy of nasopharyngeal carcinoma. Methods X-ray radiation intermittently several established nasopharyngeal carcinoma cell line CNE 2 radiation resistant cells CNE 2R, by BioStarH 141s CNE 2 gene microarray of differentially expressed genes with the CNE 2R. Results CNE 2 cells and CNE 2R differentially expressed genes 308, 176 increase, down 132. in the differentially expressed genes, 76-bit point of difference appears more than 6 times, of which 36 were down, 40 up-regulated, including DNA repair-related, cell cycle, apoptosis, cytoskeletal proteins, cell proliferation, metabolism, protein synthesis, signal transduction, immunity, etc. related genes, gene distribution is mainly the more obvious changes in DNA repair genes, cytoskeleton, cell cycle and apoptosis-related genes. Conclusion CNE 2 cells resulting from repeated exposure to radiation resistant cells occurs at the genetic level, some mutant through the regulation of genes of which it is possible to regulate cellular radiosensitivity. [Keywords:] nasopharyngeal cancer, gene chip, radiation resistant ABSTRACT: Objective To observe the differential gene expression in human nasopharyngeal carcinoma (NPC) cell line with different radiosensitivity by cDNA microarray analysis. Methods A radioresistant cell line, CNE 2R, was established from a human nasopharyngeal carcinoma cells line CNE 2 by repeated X ray irradiation. The differential gene expression of CNE 2 and CNE 2R was screened with cDNA microarray by BioStarH 141s profile gene chip. Results There were expressed 308 genes to be screened out between CNE 2R cell line with different radio