Radon exposure on rat tracheal - the impact of bronchial epithelial cell transformation.doc

 PAGE \* MERGEFORMAT 9 Radon exposure on rat tracheal - the impact of bronchial epithelial cell transformation The living environment of human natural background radiation, due to inhalation of radon and its daughters dose of radiation generated by about 54% of the health hazards caused by radon exposure has become a research hotspot in recent years. Rat tracheal - bronchial epithelial cells is to study the biological effects of radon exposure to good models, but due to the exposure devices conditions, in the past many in vitro studies. In this study, the use of multi-functional mobile radon chamber dynamic inhalation exposure in rats, using established animal models, using brushing method after digestion of rat isolated trachea - bronchial epithelial cells [1], observation of the target cells in vitro after exposure to radon occurrence transformation process, explore the radon exposure to the damaging effects on cells. 1 Materials and methods 1  1 treatment of laboratory animals experimental Wistar male rats, 7 to 8 weeks of age (Shanghai Experimental Animal Slack limited liability company) Permit No.: SCXK (Shanghai) 2003-0003. Using self-made HD-3 multi-function mobile radon chamber, dynamic inhalation exposure in rats. In accordance with the cumulative exposure to radon exposure, divided into 60,90,120 work-months (Working Level Month, WLM) and the control group a total of four groups of six animals. Daily exposure time 8h, weekly 6d, animal free movement during the food poisoning. 1  2 selective medium for the preparation of selective F12 medium Ham’s F12 medium containing 2% fetal calf serum, 5mg / L insulin, 0  3μ mol / L hydrocortisone. 1  3 trachea - bronchial epithelial cells isolation and culture of the experimental group and control group rat tracheal - bronchial epithelial cells isolation and culture using established in our laboratory after digestion brushing method, see reference [1]. 1 