Rapamycin combined with cisplatin in ovarian cancer SKOV3 cells proliferation.doc

Rapamycin combined with cisplatin in ovarian cancer SKOV3 cells proliferation.doc

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Rapamycin combined with cisplatin in ovarian cancer SKOV3 cells proliferation

 PAGE \* MERGEFORMAT 15 Rapamycin combined with cisplatin in ovarian cancer SKOV3 cells proliferation Study: Duan Zhao, Yang Xuemei, Xinglan Ying, Pan Yanyan, WANG Yanhua, Li Hong [Abstract] Objective To investigate rapamycin alone and in combination with cisplatin on the human ovarian cancer SKOV 3 cell growth and the possible molecular mechanism. Methods Cultured human ovarian cancer SKOV 3 cells in the experimental group with different concentrations of rapamycin were adriamycin, cisplatin, and 40nmol / L rapamycin +10 mol / L cisplatin, MTT assay of these drugs inhibit the rate of cell proliferation; flow cytometry analysis of apoptosis and cell cycle distribution. Results rapamycin alone and in combination with cisplatin could significantly inhibit the application of human ovarian cancer SKOV 3 cell proliferation, its role in present time and dose dependent, and the combined effects of both drugs, the inhibition rate was significantly higher (P lt;0.05), showed the two drugs synergistic effect; flow cytometry demonstrated that rapamycin SKOV 3 ovarian cancer can occur G1 arrest, and can induce apoptosis, the apoptosis rate increased with drug concentration. Conclusion rapamycin cis Platinum can significantly inhibit human ovarian cancer SKOV 3 cell proliferation, cell cycle arrest and induction of apoptosis, and when combined the two drugs showed synergy. [Keywords:] rapamycin; cisplatin; ovarian cancer SKOV 3 cells; cell cycle; cell apoptosis ABSTRACT: Objective To study rapamycin alone or in combination with cisplatin on the growth of human ovarian cancer SKOV 3 cells and the possible molecular mechanism. Methods Human ovarian cancer SKOV 3 cells were cultured in vitro. The experimental group was administered with different concentrations of rapamycin and cisplatin, and 40nmol / L rapamycin +10 mol / L cisplatin. MTT assay was applied to examine these drugs inhibition rate on cell proliferation; flow cytometry was used to analyze the apoptos

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