Recombinant human interferon-γ on retrobulbar fibroblasts CD40 mRNA expression in.docVIP

 PAGE \* MERGEFORMAT 2 Recombinant human interferon-γ on retrobulbar fibroblasts CD40 mRNA expression in [Keywords:] Fibroblast;, thyroid-associated ophthalmopathy;, interferon, recombinant;, antigen, CD40;, cells, cultured Abstract: Objective To study the recombinant human interferon γ (rhIFNγ ) under the action of normal and thyroid-associated ophthalmopathy (TAO) in patients with retrobulbar fibroblasts (RF) CD40 mRNA expression. Methods RTPCR method rhIFNγ different concentrations and 400 U / mL rhIFNγ different time in vitro induced by normal and TAO in patients with RF CD40 mRNA by semi-quantitative analysis. Results 100,200,400,1000 U / mL rhIFNγ role of the 48 h after the RF CD40 in patients with normal and TAO optical density ratio with the respective control group, the difference was statistically significant (P amp;lt;0.01); both the same concentration of Comparing the difference was not statistically significant (Pamp;gt; 0.05). The 400 U / mL rhIFNγ role 12,24,48,72 h, the normal and TAO in patients with RF CD40 ratio of optical density compared with the respective control group, the difference was statistically significant (P amp;lt;0.05), compare the difference between the two at the same time There was no significant (Pamp;gt; 0.05). Conclusions Patients with RF were normal and TAO expression of CD40 mRNA. rhIFNγ can up-regulate normal and TAO in patients with RF CD40 mRNA levels, a concentration-and time-dependent. Keywords:: fibroblast; thyroid-associated ophthalmopathy; interferon, recombinant; antigen, CD40; cells, cultured ABSTRACT: Objective To quantify the mRNA expression of CD40 induced by recombinant human interferon gamma (rhIFNγ ) in cultured human retroocular fibroblast (RF) from normal subjects and thyriod associated ophthalmophy (TAO) patients. Methods Half quantifying the mRNA expression of CD40 induced by different concentration and time rhIFNγ in cultured human RF from normal subjects and TAO patients. Results The abs