Recombinant human thymosin 1 gene expression in hairy roots of Astragalus.docVIP

 PAGE \* MERGEFORMAT 10 Recombinant human thymosin 1 gene expression in hairy roots of Astragalus Study: Fan Wei Han Shuhai inclusive Xiao Liu Yonggang Zhang Honggui group [Abstract] Objective hairy roots of Astragalus expression of human thymosin @ 1. Synthesized human prothymosin @ 1 gene, the recombinant vector pCAMBIA1301 hT @ 1, the astragalus hypocotyls explants mediated by Agrobacterium rhizogenes C58C1 lead into the hairy roots of Astragalus, hygromycin resistance obtained hairy roots by PCR, human prothymosin @ 1 gene integration, SDS PAGE, Western blot analysis of human prothymosin @ 1 gene expression. Results tide mold Integration of hairy roots and resistance factors expressed human prothymosin @ 1 gene. concluded that the recombinant human prothymosin @ 1 gene in hairy roots of Astragalus can be expressed, as in hairy roots of Astragalus bioreactor @ industrial production of human prothymosin 1 provides an experimental basis. [Keywords:] human prothymosin @ 1, hairy roots of Astragalus, Agrobacterium rhizogenes, the recombinant vector Human prothymosin @ 1 (human thymosin alpha1, hT @ 1) is a potent immune enhancer, mainly for autoimmune liver disease, primary immunodeficiency, chronic hepatitis B, hepatitis C, melanoma, AIDS and certain cancers (breast, lung, liver, gastrointestinal tract cancer, malignant melanoma, Hodgkin lymphoma, non-Hodgkin’s lymphoma, kidney cancer, etc., male infertility, atherosclerosis sclerosis and other immune deficiency diseases, immunosuppressive diseases and the treatment of viral diseases and cancer, with good clinical outcome (1,2). In this study, construct plant binary expression vector pCAMBIA1301 hT @ 1, then rhizogenes C58C1 mediated hairy root of Astragalus into the final expression in hairy roots of Astragalus human prothymosin @ 1, so as to establish the hairy roots as a bioreactor hT @ 1 method. 1 Materials and methods 1.1 Materials Seed provided by the Institute of Grassland in China