RNA interference inhibition of expression of cisplatin-induced survival of colon cancer cell line HCT116 apoptosis.doc

 PAGE \* MERGEFORMAT 10 RNA interference inhibition of expression of cisplatin-induced survival of colon cancer cell line HCT116 apoptosis [Abstract] Objective To investigate survivin (survivin) interference increased HCT 116 colon cancer cell lines to cisplatin (CDDP) sensitivity of the mechanism. Methods in HCT 116 cells transfected with lipofectamine 2000 survivin, siRNA sequence; fluorescence quantitative PCR Detection of survivin mRNA expression; Western blot the expression of survivin protein; detected by flow cytometry apoptosis. Results of the siRNA transfection of survivin was significantly reduced survivin protein expression; transient transfection of survivin siRNA sequence HCT 116 cells to low concentrations (1.0 g / ml) under the action of CDDP, the apoptosis rate was significantly higher than the negative control group. Conclusion interference of survivin downregulation of survivin can induce apoptosis, increase colon cancer cell line HCT 116 CDDP sensitivity. [Keywords:] Survivin; siRNA; cell apoptosis Survivin (survivin) inhibitor of apoptosis protein family is the smallest member (1), because of its strong inhibition of apoptosis, in cancer research has important value (2). Survivin overexpression in colon cancer, and development of colon cancer play an important role in the process (3,4). Recent studies have found that survivin and resistance to anticancer drugs is closely related to (5). Therefore, the experiments using RNA interference technology to reduce survivin in colon cancer cell lines HCT 116 in the expression of survivin expression detected in low concentrations inhibit chemotherapy drug cisplatin (CDDP) sensitivity of colon cancer cells to cytotoxic drugs is not sensitive to the production of CDDP effects and possible mechanisms. 1 Materials and methods 1.1 Reagents DMEM, Trizol was purchased from Invertrogen company. FBS purchased from Evergreen Company. AnnexinV PI apoptosis detection kit, protein quantitation kit,