RNA interference inhibition of expression of cisplatin-induced survival of colon cancer cell line HCT116 apoptosis.docVIP

 PAGE \* MERGEFORMAT 10 RNA interference inhibition of expression of cisplatin-induced survival of colon cancer cell line HCT116 apoptosis [Abstract] Objective To investigate survivin (survivin) interference increased HCT 116 colon cancer cell lines to cisplatin (CDDP) sensitivity of the mechanism. Methods in HCT 116 cells transfected with lipofectamine 2000 survivin, siRNA sequence; fluorescence quantitative PCR Detection of survivin mRNA expression; Western blot the expression of survivin protein; detected by flow cytometry apoptosis. Results of the siRNA transfection of survivin was