RNAi inhibition of endogenous gene expression in the Study of Liver Diseases.doc

 PAGE \* MERGEFORMAT 16 RNAi inhibition of endogenous gene expression in the Study of Liver Diseases Author: Zhao-fu Zhang Guoying Yang Hui Liu social Han 5 [Keywords:] RNA interference technology Keywords: RNA interference; endogenous gene; TNF-α ; Fas; caspase-8 RNA interference (RNA interference, RNAi), or with the nucleotide composition of a short double-stranded RNA (dsRNA) to replace the traditional antisense nucleic acid to post-transcriptional gene silencing (post-transcriptional gene silencing PTGS). Since 2001, 2002, for two consecutive years by the United States ‘Science’ magazine of the year, one of 10 major breakthrough in technology since the 2003 ~ 2004 continued to heat high, and have been quickly and widely applied to gene function, gene expression and regulation mechanism and other popular areas, and for gene therapy has opened up new avenues. RNA interference technology, with its high degree of specificity and effectiveness of functional genomics studies, is considered a very useful tool. In medical research, it is not only effective in inhibiting the expression of exogenous genes, showing a good anti-virus applications, but can be very specific to silence endogenous gene expression, as we pass selectively inhibit gene expression of harmful to study the anti-inflammatory, anti-apoptosis, anti-tumor therapeutic strategies such as providing new ideas and methods [1]. 1 RNA interference mechanism and technical principles When the virus genes into human genes, transposon-derived genes loaded with random integration into the host cells genome, and transcription using the host cells often produce some dsRNA. Host cells respond quickly to these dsRNA, its cytoplasm in the endonuclease Dicer to dsRNA cut with a specific length and structure of several small fragments of RNA (approximately 21bp ~ 23bp), or siRNA. siRNA in the cell the role of RNA helicase melting into the next justice chain and antisense chain, followed by anti-sense si