RP-HPLC determination of election in the hands of No. 1 buckwheat of inositol.docVIP

 PAGE \* MERGEFORMAT 7 RP-HPLC determination of election in the hands of No. 1 buckwheat of inositol Study: Heart Peng sickle, hook Qiufen, Wu Bing, Zhao Gang [Abstract] Objective To establish the election in the hands of No. 1 buckwheat determination of inositol. Methods NH2 column (4.6 mm 250 mm, 5 m, column temperature was 30 . 70% acetonitrile mobile phase at a flow rate of 1.0 ml / min. Results chiral inositol in the 1.340 ~ 8.040 g, peak area and sample size with a good linear regression equation A = 65 017 C-6 019, r = 0.999 5 (n = 6), the average recovery was 97.42% . buckwheat No. 1 selection in the hands of the inositol content of 2.9 mg / g. Conclusion The method is simple, accurate, reproducible and can be used to select the quality control of buckwheat 1. [Keywords:] buckwheat, chiral inositol, content, HPLC Abstract: ObjectiveTo develop an RP-HPLC method for determination of D-Chiro-Inositol (DCI) in Xuanqiao 1. MethodsThe analysis was carried out at 30 on a NH2 column eluted with a mobile phase consisting of a mixture of acetonitrile-water ( 70:30). The flow rate was 1.0 ml / min. ResultsThe calibration curve was linear within the concentration ranges of 1.340 ~ 8.040 g (r = 0.999 5), the average recovery was 97.42%, and the DCI in Xuanqiao 1 was 2.9 mg / g. ConclusionThis method is simple, accurate, replicate and suitable for determination of DCI in Xuanqiao 1. Keywords:: Fagopyrum tataricum; D-Chiro-Inositol; Assay; RP-HPLC Buckwheat F.tataricum (L. Gaerth. Genus Polygonaceae, buckwheat genus (Fagopyrum dicotyledonous grain crops, it is the unique medicinal and edible plants, with a high nutritional value and medicinal value. Buckwheat contains a large number of flavonoids (flavonoida, modern medical research showed that buckwheat has antioxidant, lower blood sugar, blood fat, anti-tumor and other pharmacological activities [1], recent studies have shown that buckwheat contains maltitol, chiral inositol (D-chiro- inostiol,