RP-labeled oligonucleotide ion pair chromatography_0.docVIP

 PAGE \* MERGEFORMAT 18 RP-labeled oligonucleotide ion pair chromatography Study: High-Yun Li Peng Jing Hua Wu Liqing Shengling Hui Fu Boqiang [Abstract] labeled oligonucleotide established reversed-phase ion pair chromatography method to optimize the mobile phase concentration of acetic acid and triethylamine (0 ~ 0.15 mol / L, pH 4.5 ~ 7.0 and the elution strength of chromatographic conditions. On the 5 mer , 10 mer and 15 mer non-labeled and 5 ‘carboxy fluorescein (5’FAM labeled oligonucleotide comparative analysis of the reservation to study the fluorescent oligonucleotide reten