Rivanol different germ layers of cell proliferation.doc

 PAGE \* MERGEFORMAT 14 Rivanol different germ layers of cell proliferation [Abstract] Objective: To observe the rivanol from different germ layers of cell growth and proliferation. Methods: Using cell morphology was observed by different concentrations of rivanol (3.13,6.25,12.5 g ml-1 ) for different time (24,48,72 h) morphological changes after the same time, cells were observed under a fluorescence microscope in the fluorescence intensity of rivanol. using thiazolyl blue (MTT) method and the silver staining nucleolar organizer region (AgNOR ) after testing the role rivanol change the cell proliferation. Results: rivanol After 24 h, the liver cells (BRL 3A) and fibroblasts (L929) had no obvious morphological changes in human umbilical vein endothelial cells (HuVEC ) or even disappear thinner processes, cell growth slows down; with the increase of drug concentration, cell number per unit area decreased; 3 cells rivanol autofluorescence increased with time and prolonged drug concentration is enhanced. MTT results rivanol proliferation of 3 have some inhibitory effect, in which the ability to suppress cell proliferation HuVEC stronger than the other two cells (both P lt;0.01). rivanol at different concentrations under, HuVEC nucleolar organizing area than the other two cells reduce the rate of more obvious difference was statistically significant (P lt;0.05 or P lt;0.01). Conclusion: the source rivanol 3 different germ layers inhibited cell proliferation, in which the HuVEC In particular, a significant inhibition, suggesting the drug on the inhibition of endothelial cell proliferation is more specific. [Keywords:] rivanol; human umbilical vein endothelial cell; liver cells; fibroblasts [Abstract] Objective: To observe the influence of rivanol on growth and proliferation of cells form different germ layers. Methods: After treated by different rivanol concentration (3.13,6.25,12.5 g ml-1) and different work time (24,48 , 72 h), morphological changes of