RPHPLC determination of andrographolide in Andrographis paniculata extract the content of.doc

 PAGE \* MERGEFORMAT 9 RPHPLC determination of andrographolide in Andrographis paniculata extract the content of [Abstract] Objective To establish the Andrographis extract method for determination of andrographolide. Methods RP-HPLC, column: Hypersil ODS C18 (4.0 mm * 250 mm, 5 μ m), mobile phase: acetonitrile  volume fraction of 0.1% phosphoric acid solution (volume ratio 25:75), detection wavelength 226 nm a flow rate of 1.0 mL / min. Results andrographolide at 0.5 ~ 5.0 μ g (r = 0.9995) the scope of a good linear relationship, the average recovery was 98.73%, RSD was 1.32%. Conclusion The method is simple, accurate, reproducible, can be used as Andrographis paniculata extract method for determination of andrographolide. [Keywords:] RP-HPLC; Andrographis extract; andrographolide Andrographolide (andrographolide), also known as CSC B element, is Acanthaceae plant Andrographis Andrographis paniculata (Burm.f.) Nees, one of the active ingredient, has anti-bacterial, anti-inflammatory, relieving cough and asthma, heat-clearing and detoxifying, Liangxue swelling and other role, is widely used in upper respiratory tract, gastrointestinal tract bacterial infection caused by various diseases [1]. About Andrographis paniculata andrographolide and its preparation in the content analysis method has been determined by colorimetry, TLC, HPLC and other reports [2-5]. In this paper, reversed-phase high performance liquid chromatography (RP  HPLC) determination of andrographolide in Andrographis paniculata extract the content, method is simple, rapid, accurate, and can be used as Andrographis paniculata extract method for determination of andrographolide. An instrument and reagent U.S. production of HP1100 Agilent Technologies Co., high-performance liquid chromatography, G1311A quaternary pump, G1314A UV detector, HP1100/Agilent ChemStation, Lambda25 UV spectrophotometer (with scanning), Germany BP211D Sartorius electronic balance ( a sense of the amount of 0.01