三分类血细胞沸轮析仪白细胞分类与手工法结果比较.doc

三分类血细胞分析仪白细胞分类与手工法结果比较 曾振炜 【摘要】目的 探讨日本东亚公司Sysmex KX-21三分类血细胞分析仪白细胞分类计数与手工法的方法学差异。方法 通过对中山二院门诊检验室随机抽取的87例普通门诊病人的标本进行白细胞分类仪器法与手工法测定，得出两组白细胞分类结果，并进行统计分析。结果 87例普通门诊病人血白细胞分类结果显示：中性粒细胞与淋巴细胞计数两种方法无明显差异（P0.05），中间型白细胞计数两种方法有显著性差异(P0.01),仪器法明显高于手工法.中性粒细胞、淋巴细胞与中间型白细胞计数两种方法的相关系数分别为0.8510、0.8373及0.5072。结论 Sysmex KX-21三分类血细胞分析仪中间型白细胞计数与手工法存在较大的方法学差异，不能作为白细胞分类常规过筛试验指标。 【关键词】血细胞分析仪；白细胞分类；手工法 Compare the results between the trisection Automatic Hematology analyzer and the manual method Zeng zhenwei 【Abstract】Objective To study the methodological difference between the instrument of the trisection automatic Hematology analyzer Sysmex KX-21 produced by East Asia Japan Company and the manual method. Methods The 87 blood samples collected from the out-patient department of The Second Affiliated Hosptil of Sun Yat-sen University were analyzed with the instrument of Sysmex KX-21 Automatic Hematology analyzer and the manual method.The results were compared and analyzed. Results The 87 blood samples’ leukocyte classified results showed that W-SCR and W-LCR count were not different between the two methods(P0.05),but there was significant difference in W-MCR count between the two methods(P0.01).The results of Sysmex KX-21 was apparently higher than that of the manual method.The correlation between the results from Sysmex KX-21 and the manual method was 0.8510,0.8373,0.5072 respectively for W-LCR,W-SCR,W-MCR Conclusion There was significant difference in W-MCR count between the instrument of Sysmex KX-21 Hematology analyzer and the manual method,so the instrument of Sysmex KX-21 can’t be the guide line of general filtration for leukocyte classification. 【Key Words】Hematology analyzer;leukocyte classification;the manual method 目前，许多大、中、小医院使用各种类型的全自动血细胞分析仪进行白细胞二分类、三分类甚至五分类计数。以三分类全自动血细胞分析仪的应用较普遍。日本东亚公司的Sysmex KX-21三分类血细胞分析仪是根据电阻抗变化获得电脉冲信号而将白细胞分为三大类：小型白细胞（W-SCR）、中间型白细胞（W-MCR）和大型白细胞（W-LCR）。其中中间型白细胞包括嗜酸性粒细胞、嗜碱性粒细胞、单核细胞及原始细胞或幼稚细胞。由于该仪器对中间型白细胞的分辨功能有限，与人工显微镜分类可能存在较大的差异。三分类血细胞分析仪对白细胞分类可否作为常规过筛试验指标，很