家兔小肠平滑肌实验报告.doc

家兔小肠平滑肌实验报告 医学机能学实验—PH、AD、Ach等因素对离体家兔肠肌运动的影响 PH、AD、Ach等因素对离体家兔肠肌运动的影响 XXX XXXXX大学第二临床医学院 临床医学 （2）班 第7组 20072090211 【摘要】：目的：通过观察HCl、NaOH、23℃台试液、肾上腺素和乙酰胆碱对离体家兔肠肌的激动作用，加深理解各药物的激动和阻断作用。方法 在保证个影响因素不相互干扰的情况下，逐步给予相应的刺激和给药，用微机生物信号采集处理系统观察各项处理前后的收缩幅度变化，分析探讨各种理化刺激引起循环、呼吸、泌尿等功能的适应性改变。结果 得到了各项处理前后的收缩幅度原始图表。结论 滴加HCl、23℃台试液、肾上腺素，肠肌标本的收缩张力下降；滴加NaOH、乙酰胆碱，肠肌标本的收缩张力升高；其中使用23℃台试液的肠肌标本紧张性上升。[1] Hcl, NaOH,by observation naoh, 23 ℃ #39;s try and that of epinephrine and b, as alkali to the rabbits from the muscles of excitement, comprehension of drugs and stops. Method In a way to ensure that factors influencing not interfere with each other cases, has given the appropriate incentives and drug, with microcomputers biological signal collecting systems and observe the processing of the amplitude variation, which caused by various rationalized cycle, breathing and urinary for functions change. Results Received the attention of the contraction in the diagram. the conclusion was added, one twenty-three ℃ hcl try and that of epinephrine. the specimen the contraction of muscle tension ； drop down with naoh, even terror; the muscles of the increase of tension ； used 23 ℃ #39;s try the sausage in the availability of increased tension. 【关键词】：离体肠肌；台氏液；酸碱度；肾上腺素 肾上腺素：由肾上腺髓质分泌的一种儿茶酚胺激素。在应激状态、内脏神经刺激和低血糖等情况下，释放入血液循环，促进糖原分解并升高血糖，促进脂肪分解，引起心跳加快。[1] 【Key words】：Intestinal muscle from the body; Tyrode#39;s Salts Solution;PH;AD Adrenaline;epinephrine：One kind of catecha phenol amine hormone which secretes by the adrenal medulla. In situations and so on stress condition, splanchnic nerve stimulation and hypoglycemia, releases into the blood circulation, the promotion glycogen decomposes and elevates the blood sugar, the promotion steatolysis, causes the palpitation to speed up. 【引言】消化道平滑肌与骨骼肌、心肌一样，具有肌肉组织共有的特性如兴奋性、传导性、收缩性，但是表现出与骨骼肌、心肌所不同的特点，既兴奋性低、收缩缓慢、富有伸展性、具有紧张性、自动节律性、对化学、温度和机械牵张刺激较敏感等。 为观察哺乳动物消化道平滑肌的特性，必须给予离体肠肌以接近于在体情况的适宜环境。本实验以台氏液作灌流液。其离子成分和pH与哺乳动物的体液相似。在整个实验过程中，灌流液的温度恒定在38℃左右并每隔20分钟更换一次灌流液，持