赤芍水提物对肝星状细胞株 HSC.docVIP

　　赤芍水提物对肝星状细胞株 HSC 作者：韩海啸 李军祥 刘大新 朱陵群 崔巍 【摘要】 目的应用流式细胞法，研究灌服赤芍水提物后的犬血清对乙醛造模后的肝星状细胞株 HSC-T6细胞的促凋亡作用。方法采用乙醛造模后的肝星状细胞株 HSC-T6 作为体外研究模型，以赤芍水提物给彼格犬一次性灌胃，取给药前的血清、给药后 1 h、2 h、3 h、5 h 血清作为实验药物血清。以流式细胞法（floetry）分别检测以上采血时间点上 2% 浓度的药物血清对乙醛造模后的肝星状细胞株 HSC-T6 作用 72 h 后的促凋亡作用。结果 2 h、3 h 两个时间点的含药血清能明显促进 HSC-T6 细胞的凋亡，其中 2 h 2% 含药血清的促凋亡率 5.71%，3 h 2% 含药血清的促凋亡率 5.73%。和同样条件下用空白血清培养的 HSC-T6 细胞相比其凋亡 比例显著性增加（均为 P lt; 0.05）。在药物血清的作用下两个时点组的 HSC-T6 细胞在 G0/G1 期细胞比例明显上升（P lt; 0.05）；而 S 期细胞显著减少（P lt; 0.05）；但 G2/M 期并没有一致性的变化。结论 两个时点的药物血清对 HSC-T6 细胞都有显著的促凋亡作用，且能显著增加 HSC-T6 细胞在 G0/G1 期的比例及显著降低 S 期的比例，这可能是其抑制 HSC-T6 细胞增殖及促凋亡的原因之一。 【关键词】 赤芍水提物；肝星状细胞株 HSC-T6；凋亡； 酒精性肝纤维化；细胞培养；流式细胞术 〔Abstract〕 Objective By means of floetry, oting effects of the dog serum fed by Chishao distilled substances on hepatic stellate cell (HSC-T6) builded model by acetaldehyde. Methods The experiments adopted eternalized human hepver Fibrosis; Cell Culture; Floetrystellate cell (HSC-T6) builded model by acetaldehyde, for study in vitro. Feeding the dog e, before feeding and at 1-hour、2-hour、 3-hour and 5-hour different points of time, edicated serums had been proved best inhibiting effects, after feeding as the medicated serum for experiment. By means of floetry, ined the apoptosis-promoting effects in 72 hours on HSC-T6 cells by 2% concentration of medicated serum at different points of time. Results The medicated serums has significant effects of promoting HSC-T6 apoptosis at these te. pared edicated serums under the same conditions, the proportion of apoptosis had a significant increase (P lt; 0.05). At the 2-hour, 3-hour point, the apoptosis rate of it is 5.71%、5.73%. HSC-T6 cells, proportion s had significant promoting-apoptosis effects on HSC-T6. Dramatically decreasing HSC-T6 cells proportion in S period and increasing it in the G0/G1 period oting thEir apoptosis. 〔Key P-1 mRNA 等[1]。常规复苏、传代后，在 5% CO2 饱和条件下细胞培养箱内培养待用。 1.1.2 药品和主要试剂 ①赤芍水提物制备：赤芍水煮提取，提取液经 50% 乙醇沉淀后，滤除沉淀，上清液回收溶剂得赤芍水提物。1 mL 水提物中含 3 g 生药，由北京中医药大学东方