KnockOutSRUserGuide-ThermoFisherScientific.pdfVIP

KnockOut™ SR Description KnockOut™ Serum Replacement (KnockOut™ SR) is a more defined, FBS-free formulation designed to directly replace fetal bovine serum (FBS) in supporting the growth and derivation of human and other mammalian embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) on fibroblast feeder cells. KnockOut™ SR also supports cryopreservation, embryoid body formation, and in vitro differentiation studies. For mouse ESCs, KnockOut™ SR can replace FBS in media used for blastocyst injection, embryo culture, electroporation or cationic lipid transfection, clone selection, cryopreservation, derivation of new ESC lines, embryoid body formation, and in vitro differentiation studies. By providing the documentation required for regulatory review, KnockOut™ SR enables you to reduce your burden in qualifying reagents when transitioning from research applications to clinical applications. Each container is sterile filtered. Product Catalog No. Amount Storage Shelf life* 10828-010 100 mL ™ –20°C to –5°C; Protect from light 18 months KnockOut Serum Replacement 10828-028 500 mL *Shelf life duration is determined from the Date of Manufacture. Product use • KnockOut™ SR is stable for up to 4 weeks at 2°C to 8°C, For human ex vivo tissue and cell culture processing applications. protected from light. Caution: When used as a medical device, Federal Law restricts • Working volumes can be aliquoted and stored at this device to sale by or on the order of a physician.