南京农业university生物化学教学课件23.pptVIP

The binding-change model was elegantly supported by two other experimental observations X-ray crystallography revealed that the three b subunits of F1 assumes three different conformations, with bound ADP, ATP analog, or empty respectively. Rotations of the g subunit and the c -ring of the F1 unit in three discrete steps of 120o (as being powered by ATP hydrolysis catalyzed by the b subunits) have been directly observed using fluorescence microscopy (1997) g g g Boyer Rod-shaped g subunit. ADP App(NH)p Empty Structure determination indicates that indeed the three b subunits are inequivalent