Characterization of the Vaginal Micro- and Mycobiome in Asymptomatic Reproductive-Age Estonian Women.docVIP

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2017-05-07 发布于上海
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Characterization of the Vaginal Micro- and Mycobiome in Asymptomatic Reproductive-Age Estonian Women.doc

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Characterization of the Vaginal Micro- and Mycobiome in Asymptomatic Reproductive-Age Estonian Women

CharacterizationoftheVaginalMicro-andMycobiomein AsymptomaticReproductive-AgeEstonianWomen TiinaDrell1,2*,TriinLillsaar1,2,LeaTummeleht1¤,JaakSimm1,AnuAaspo?llu1,EddaVa¨in3,IvoSaarma3, AndresSalumets2,4,5,GilbertG.G.Donders6,7,MadisMetsis1,2 1CentreforBiologyofIntegratedSystems,TallinnUniversityofTechnology,Tallinn,Estonia,2CompetenceCentreonReproductiveMedicineandBiology,Tartu,Estonia, 3FertilitasHospital,Haabneeme,Estonia,4DepartmentofObstetricsandGynaecology,UniversityofTartu,Tartu,Estonia,5InstituteofBiomedicine,UniversityofTartu, Tartu,Estonia,6DepartmentObstetricsandGynecology,RegionalHospitalHHart,Tienen,Belgium,7Femicarevzw,ClinicalResearchforWomen,Tienen,Belgium Abstract Theapplicationofhigh-throughputsequencingmethodshasraiseddoubtintheconceptoftheuniformhealthyvaginal microbiota consisting predominantly of lactobacilli by revealing the existence of more variable bacterial community composition.Asthisneedstobeanalyzedmoreextensivelyandthereislittlestraightforwarddataregardingthevaginal mycobiomeofasymptomaticwomenweaimedtodefinebacterialandfungalcommunitiesinvaginalsamplesfrom494 asymptomatic, reproductive-age Estonian women. The composition of the vaginal microbiota was determined by amplifyingbacterial16SrRNAandfungalinternaltranscribedspacer-1(ITS-1)regionsandsubsequentlysequencingthem using454LifeSciencespyrosequencing.Wedelineatedfivemajorbacterialcommunitygroupswithdistinctivediversityand species composition. Lactobacilli were among the most abundant bacteria in all groups, but also members of genus Gardnerellahadhighrelativeabundanceinsomeofthegroups.MicrobialdiversityincreasedwithhighervaginalpHvalues, and was also higher when a malodorous discharge was present, indicating that some of the women who consider themselveshealthymaypotentiallyhaveasymptomaticbacterialvaginosis(BV).Ourstudyisthefirstofitskindtoanalyze themycobiomethatcolonizesthehealthyvaginalenvironmentusingbarcodedpyrosequencingtechnology.Weobserved 196fungaloperationaltaxonomicunits(