20_s_人参皂苷rg3对卵巢癌生长抑制作用的实验研究.pdfVIP

108 2007 2 23 2 :1005 - 2216(2007)02 -0108 - 03 20(S)- Rg3 1 1 1 2 许天敏 ,崔满华, 谷丽萍,李慧景 【　】　　观察 20(S)-人参皂苷 Rg3对卵巢癌细胞株 SKOV3及卵巢癌皮下移植瘤 长的抑制作用并探 讨其机制。　2005-02 -2006-04在吉林大学第二医院, 通过体外培养绘制细胞 长曲线,观察 Rg3对卵巢癌 细胞株 SKOV3 长的影响。通过建立卵巢癌皮下移植瘤动物模型及 PCNA(增殖细胞核抗原)免疫组织化学染 色, 来观察 Rg3 对移植瘤的 长抑制作用, 并利用新 血管计数实验检测 Rg3对卵巢癌组织血管 成的影响。 　Rg3对卵巢癌细胞株 SKOV3 长具有抑制作用, 随着其浓度的增加抑制作用增强。在卵巢癌皮下移植瘤 动物模型中, 注射 Rg3试验组瘤重及瘤体积明显低于对照组, PCNA的表达随着 Rg3的浓度增加而降低。 Rg3组 肿瘤周围的血管数明显少于对照组。　Rg3可明显抑制体外培养卵巢癌细胞株 SKOV3及卵巢癌皮下移植 瘤的 长, 其机制可能是通过抑制肿瘤内血管 成而发挥作用。 【】　卵巢癌;人参皂苷;肿瘤 长 :R71　　:A Inh ib itory e ffects of 20(S )-ginsenoside R g3 on ovarian cancer grow th. 　　XU Tian-m in, CUIMan-hua, GU L-i ping, et a.l 　　TheD eparmt entof Gynecology andObstetrics, TheSecond ospitalof Jilin University, Changchun 130041, China Ab strac t　O b ject iv e　 o study the effects of 20(S)-ginsenoside Rg3 on ovarian cancer grow th and its mechan ism. M eth ods　From Feb. 2005 to Apr. 2006 in the the Second Hosp ital of Jilin University, the grow th curve, the model of o- varian cancer and PCNA imm unohistochem ical staining w ere u sed to observe the effects of 20(S)-ginsenoside Rg3 on o- varian cancer grow th. hem echanism sw ere analysed by assay of tum o-r induced angiogenesis. R esu lts　In vivo, we di-s covered that the averagew eight and the volum e of ovarian cancer in each Rg3 groupw as lower than those of control group. he PCNA mi munohistochem ical staining showed that PCNALI decreased w ith the increasing of Rg3 concentration and the number of vessels oriented tow ard the tum or m ass was less than that in the untreated contro.l In vitro, the resu lts di-s p layed that Rg3 also inh ibited the grow th of SKOV3 cells. Conc lu sion　20(S)-ginsenoside Rg3 can obviously inh